Generator

Part:BBa_K3765021:Design

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)


OpdA+SpyTag002+sfGFP with OpdA signal peptide under T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2181
    Illegal SpeI site found at 45
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2187
    Illegal SpeI site found at 45
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2009
    Illegal BamHI site found at 2169
    Illegal XhoI site found at 2151
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2181
    Illegal SpeI site found at 45
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2181
    Illegal SpeI site found at 45
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1371
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein generator contains SpeI and XbaI restriction sites from the vector pETMCN. We used Gibson Assembly for cloning, so there were no hindrances in this regard. In case someone wants to use this part, it is suggested to get it synthesized and use Gibson Assembly (or other such assembly techniques) for cloning.


Source

Synthetic.

References